Nesting success of birds often is influenced by habitat features surrounding nests. However, few studies have investigated habitat influences at multiple levels and spatial scales. We examined the relationship between Blue Jay (Cyanocitta cristata) nesting success and habitat at three levels (macrohabitat, microhabitat, and nest site) and at three different scales within the microhabitat level (3.1, 0.3, and 0.07 ha circular plots surrounding nests) in central Florida. We defined successful nests as those fledging at least one offspring. An eight-fold difference in probability of nesting success existed among seven distinct macrohabitats, with Mayfield success estimates ranging from 8 to 63%. However, no difference in number of fledglings per nest existed among macrohabitats when unsuccessful nests were excluded from the analysis. Macrohabitats in which nests were more often successful had more extensive slash pine (Pinus elliottii) forest. Logistic regression analyses indicated that slash pine canopy cover was significantly related to nesting success at all three microhabitat scales, although the tree species in which a nest was placed was unimportant to nesting success. Other microhabitat and nest-site features were unrelated to nesting success. Strong circumstantial evidence indicates predation was the major cause of nest failure, but relative abundance of one potential predator, the gray squirrel (Sciurus carolinensis), was not related to nesting success in macrohabitats or slash pine canopy cover within microhabitats. In this study, logistic regression models using habitat features measured at higher levels and larger scales had better predictive power, indicating that habitat may have influenced predation at scales larger than that of a typical nest patch.